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3d plot generated by pca  (OmicSoft Corporation)
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OmicSoft Corporation 3d plot generated by pca
Poly I:C-stimulated ILT4 − and ILT4 + BDCA-3 cDCs have unique cytokine and gene signatures . (A) Experimental design of BDCA-3 DC phenotyping. (B) BDCA-3 cDCs were cultured with Poly I:C for 18 h and then sorted into ILT4 − and ILT4 + populations. Cells were then plated without further stimulation for 18 h. Supernatants were assayed for cytokine and chemokine content by luminex analysis. P -values generated using two-tailed student’s paired t -test (95% confidence interval). Graphs represent four donors. (C) BDCA-3 cDCs were stimulated with Poly I:C for 18 h, Golgistop was then added for 6 h. Cells were surface stained with ILT3, ILT4, and CD141 and then intracellularly stained with IFN-γ and TNF-α. Data showing intracellular staining are representative of one donor out of four. (D) Genomic profiling of ILT4 − vs. ILT4 + was performed using GeneChip Human Gene 1.0 ST arrays. Principal component analysis <t>(PCA)</t> was computed <t>using</t> <t>OmicSoft</t> ArrayStudio, and a plot was generated to show the relative clustering of ILT4 − and ILT4 + . ILT4 − and ILT4 + populations were compared to each other by t -test with a threshold set for a fold change >1.5 and a P -value <0.05. ILT4 gene expression was confirmed by qPCR. (Data shown are one representative donor out of four).
3d Plot Generated By Pca, supplied by OmicSoft Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pca plot generation  (GraphPad Software Inc)
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GraphPad Software Inc pca plot generation
Poly I:C-stimulated ILT4 − and ILT4 + BDCA-3 cDCs have unique cytokine and gene signatures . (A) Experimental design of BDCA-3 DC phenotyping. (B) BDCA-3 cDCs were cultured with Poly I:C for 18 h and then sorted into ILT4 − and ILT4 + populations. Cells were then plated without further stimulation for 18 h. Supernatants were assayed for cytokine and chemokine content by luminex analysis. P -values generated using two-tailed student’s paired t -test (95% confidence interval). Graphs represent four donors. (C) BDCA-3 cDCs were stimulated with Poly I:C for 18 h, Golgistop was then added for 6 h. Cells were surface stained with ILT3, ILT4, and CD141 and then intracellularly stained with IFN-γ and TNF-α. Data showing intracellular staining are representative of one donor out of four. (D) Genomic profiling of ILT4 − vs. ILT4 + was performed using GeneChip Human Gene 1.0 ST arrays. Principal component analysis <t>(PCA)</t> was computed <t>using</t> <t>OmicSoft</t> ArrayStudio, and a plot was generated to show the relative clustering of ILT4 − and ILT4 + . ILT4 − and ILT4 + populations were compared to each other by t -test with a threshold set for a fold change >1.5 and a P -value <0.05. ILT4 gene expression was confirmed by qPCR. (Data shown are one representative donor out of four).
Pca Plot Generation, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pca plot generation/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
pca plot generation - by Bioz Stars, 2026-04
90/100 stars
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Poly I:C-stimulated ILT4 − and ILT4 + BDCA-3 cDCs have unique cytokine and gene signatures . (A) Experimental design of BDCA-3 DC phenotyping. (B) BDCA-3 cDCs were cultured with Poly I:C for 18 h and then sorted into ILT4 − and ILT4 + populations. Cells were then plated without further stimulation for 18 h. Supernatants were assayed for cytokine and chemokine content by luminex analysis. P -values generated using two-tailed student’s paired t -test (95% confidence interval). Graphs represent four donors. (C) BDCA-3 cDCs were stimulated with Poly I:C for 18 h, Golgistop was then added for 6 h. Cells were surface stained with ILT3, ILT4, and CD141 and then intracellularly stained with IFN-γ and TNF-α. Data showing intracellular staining are representative of one donor out of four. (D) Genomic profiling of ILT4 − vs. ILT4 + was performed using GeneChip Human Gene 1.0 ST arrays. Principal component analysis (PCA) was computed using OmicSoft ArrayStudio, and a plot was generated to show the relative clustering of ILT4 − and ILT4 + . ILT4 − and ILT4 + populations were compared to each other by t -test with a threshold set for a fold change >1.5 and a P -value <0.05. ILT4 gene expression was confirmed by qPCR. (Data shown are one representative donor out of four).

Journal: Frontiers in Immunology

Article Title: TLR3 Signaling Promotes the Induction of Unique Human BDCA-3 Dendritic Cell Populations

doi: 10.3389/fimmu.2016.00088

Figure Lengend Snippet: Poly I:C-stimulated ILT4 − and ILT4 + BDCA-3 cDCs have unique cytokine and gene signatures . (A) Experimental design of BDCA-3 DC phenotyping. (B) BDCA-3 cDCs were cultured with Poly I:C for 18 h and then sorted into ILT4 − and ILT4 + populations. Cells were then plated without further stimulation for 18 h. Supernatants were assayed for cytokine and chemokine content by luminex analysis. P -values generated using two-tailed student’s paired t -test (95% confidence interval). Graphs represent four donors. (C) BDCA-3 cDCs were stimulated with Poly I:C for 18 h, Golgistop was then added for 6 h. Cells were surface stained with ILT3, ILT4, and CD141 and then intracellularly stained with IFN-γ and TNF-α. Data showing intracellular staining are representative of one donor out of four. (D) Genomic profiling of ILT4 − vs. ILT4 + was performed using GeneChip Human Gene 1.0 ST arrays. Principal component analysis (PCA) was computed using OmicSoft ArrayStudio, and a plot was generated to show the relative clustering of ILT4 − and ILT4 + . ILT4 − and ILT4 + populations were compared to each other by t -test with a threshold set for a fold change >1.5 and a P -value <0.05. ILT4 gene expression was confirmed by qPCR. (Data shown are one representative donor out of four).

Article Snippet: A 3D plot generated by PCA with OmicSoft ArrayStudio across all probe sets revealed that ILT4 + cells are most dissimilar from ILT4 − cells (Figure D).

Techniques: Cell Culture, Luminex, Generated, Two Tailed Test, Staining, Gene Expression